Dakota Wesleyan University (DWU) is a four-year university in Mitchell, South Dakota. It was founded in 1885 and is affiliated with the United Methodist Church. The student body averages slightly fewer than 800 students. The campus of the university is listed on the National Register of Historic Places.

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Dakota Wesleyan teams, nicknamed athletically as the Tigers, are part of the National Association of Intercollegiate Athletics (NAIA), primarily competing in the Great Plains Athletic Conference (GPAC). They formerly competed in the South Dakota Intercollegiate Conference (SDIC). Men's sports include baseball, basketball, cheerleading, cross country, football, golf, soccer, track & field and wrestling; while women's sports include basketball, cheerleading, cross country, golf, soccer, softball, track & field and volleyball. In 2015, the men's basketball team won runner-up in the 2015 NAIA Division II Men's Basketball Tournament.